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... pGEX vectors provide all three translational reading frames beginning with the EcoRI restriction site. pGEX-1λT, pGEX-6P-1, pGEX-4T-1,.

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A trehalase from Zunongwangia sp.: characterization and improving. was pGEX-6P-1 (GE Healthcare, USA. A trehalase from Zunongwangia sp.: characterization and.

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%N 1 %P 58-64 %P 7 %O Anglais %K Vertebrata. digested with restriction enzymes EcoRI and XhoI was cloned into the EcoRI/XhoI sites of a separate plasmid pGEX-6p-1,.SDS-PAGE analysis showed recombinant truncated M' protein was highly expressed by pGEX-6p-M' and the product fusion protein GST-M' reached 45% in the total bacteria.

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